Determination of diethylstilbestrol in water samples by salting-out assisted liquid-liquid extraction-UV-VIS

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International Journal of Development Research

Determination of diethylstilbestrol in water samples by salting-out assisted liquid-liquid extraction-UV-VIS

Abstract: 

A salting-out assisted liquid–liquid extraction (SALLE) technique was successfully developed  for extraction diethylstilbestrol (DES) in water samples followed by ultraviolet visible spectrophotometry (UV-Vis) analysis. Several parameters affecting the extraction, including volume of salting-out solvent, the pH of sample solution and salting-out solvent, were optimized. The optimal salting-out parameters were obtained as follows: 2 mL of acetonitrile was added to 2 mL of sample solution with pH = 3 and then 5 mL salting-out solvent containing 5 mol/L sodium chloride at a pH of 2 was added to the solution for extraction. Under optimal SALLE-UV-Vis conditions, detection limits of 0.61 μg/mL and good linearity in the range from 2.0 μg/mL to 20 μg/mL with correlation coefficient r = 0.9990 was achieved. Satisfactory recoveries ranging from 81 to 102 % were obtained with lake and tap water samples. 

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