Assessing fungal toxin sensitivity in plant-based products: an in vitro approach using lymphocyte and cytotoxicity assays

This study investigates the immunological response of plant-based products (wheat and maize) infected by Aspergillus flavus and Penicillium expansum fungal species known to produce carcinogenic and anticonvulsant toxins. Lymphocyte and cytotoxicity assays were employed to assess the sensitivity and immune response of plant cells to these toxins. The assays measured cellular viability, immune reactions, and sensitivity through the lymphocytic response to fungal metabolites. In parallel, manometer and respirometer tests were used to monitor the impact of fungal toxins on the respiratory activity of infected plant tissues, specifically examining oxygen consumption and carbon dioxide release. This integration of toxicological profiling and immunological assessments provides insights into the role of immune mechanisms in combating toxin-induced metabolic disruptions. The findings may enhance personalized toxin sensitivity profiling in agricultural products, supporting improved management strategies for fungal contamination and associated health risks.