Antimicrobial effect of pyomelanin extracted from pseudomonas aeruginosa

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International Journal of Development Research

Volume: 
07
Article ID: 
8692
4 pages
Research Article

Antimicrobial effect of pyomelanin extracted from pseudomonas aeruginosa

Zainab Radhi Abdul-Hussien and Sanaa Saeed Atia

Abstract: 

Pigmented bacterial isolates was isolated from various environmental samples. Vitek identification analysis and PCR analysis of 16SrRNA amplifying gene showed that this bacteria was related to Pseudomonas aeruginosa. Pigments was extracted with 1N HCl and ethanol.After extraction, pigment was subjected to various chemical analysis including thin layer chromatography, spectrophotometric analysis and gas chromatography –Mass spectrophotometric analysis. Chemical analysis showed that pigment was characterized as, pyomelanin which was evaluated biologically to examine its cytotoxicity against red blood cells, results showed that pyomelanin had no cytotoxic effect. Also pyomelanin was tested against pathogenic bacteria and fungi. Statistical analysisof present results showed thatpyomelanin gave an antibacterial and antifungal activity.

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