Antifungal activity of amaranthus viridis against botrytis grey mold

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Author: 
Ayesha Sadiqa, Khajista Jabeen and Sumera Iqbal
Abstract: 

AmaranthusviridisL. leaves, stem and roots were selected to find out their antifungal properties against a phytopathogenic fungus Botrytis cinereaPers. Ex Fr. Extracts of leaves, stems and roots were prepared in methanol and their different concentrations were tested against B. cinerea. All the applied conc. of leaves extract viz. 1%, 2% & 3% inhibited the growth of B. cinerea. 1% concentration was more effectively retard the dry biomass of test fungus 82.2%. Methanol extract of A. viridisroots and stems was also found effective in suppressing the growth of test fungus B.cinereaand all the concentrations of these extract cause high reduction on the colony growth of B. cinerea. Methanolic leaf extract of A. viridis is further subjected for fractional guided bioassays. Different organic fractions from leaf extract were isolated viz. n-hexane, chloroform, ethyl acetate, and n-butanol in increasing polarity order. These isolated fractions were further serially diluted to check their Minimum Inhibitory Concentration (MIC) along with a synthetic fungicide Metalaxyl + Mancozeb. The MIC was recorded for each fraction in interval of 24, 48 and 72 hours. The n-hexane, n-butanol and fungicide were found to be most effective with MIC of 0.019 mg mL-1 followed by chloroform fraction, whereas ethyl acetate was found ineffective.

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