Anti-tumor activity of newcastle disease virus strains (af 2240 and v4-upm) on aom-induced acf in sprague–dawley rats

Azoxymethane rat model is a well-established, well-appreciated, and widely used model of experimental colon carcinogenesis. Anti-tumor activity of Newcastle disease virus (NDV) strains AF2240 and V4-UPM was evaluated in-vivo to investigate the effect of different doses of NDV on the development of aberrant crypt foci (ACF) in Sprague–Dawley rats initiated with the colon carcinogen azoxymethane (AOM). The total number and crypt multiplicity as well as the degree of dysplasia of ACF were significantly decreased in treated groups with high doses of NDV compared to the groups which treated with low NDV doses. We also investigated the efficacy of the combination of NDV and 5-FU on the development of ACF in rats’ colon. RT-PCR of some colon cancer markers (COX-1and COX-2) and histopathological studies were carried out and had confirmed ACF results which showed significant decreased of ACF numbers.