Analysis of binding in an octasaccharide of siparuna guianensis aublet by methylation, and nmr studies

Siparuna guianensis Aublet is known by several popular names according to the country and / or region of distribution in Brazil. In the Cerrado regions in the states of Mato Grosso and Goiás, it is known by the name of negramina, is of the family Siparunaceae and consists of two genera, Glossocalyx and Siparuna, with approximately 65 species of shrubs and trees, except for 15 species that are trees of 20 to 40 meters high, with trunks with diameters greater than 120 cm, which usually occur in the Amazon. S. guinanensis is often cited as native to West Africa, where it is often found in areas of the types of neotropical vegetation at elevations between sea level and 3800 meters. With interest in its medicinal properties, a polysaccharide was isolated from the leaves of S. guinanensis, purified and partially hydrolyzed to obtain structural oligosaccharide. An oligosaccharide was isolated from the hydrolyzed product. At the conclusion the hydrolysis provided two monoaquats, galactose and mannose at a molar ratio of 3: 5. Methylation, Carbon-13 Nuclear Magnetic R (NMR)esonance and periodate oxidation studies indicated the presence of (1 → 4) and (1 → 6) between units of structural monosaccharides.